We then performed an unbiased comparison of clone ranking based on either titer or titer normalized to confluence (TTC).

Therefore, we developed a screening platform for high-throughput analysis of titer and confluence of etanercept-producing clones. However, no reported study so far has performed a direct head-to-head comparison of these two early reporters for predicting clone performance. Different strategies using either product titer or product titer normalized to cell number are being used to assess suspension-adapted clones when grown statically in microtiter plates. This contributes to selection of a versatile panel of clones that can be further characterized and from which the final producer clone can be selected that best fits the production requirements.Ībstract = 'The selection of clonally-derived Chinese hamster ovary (CHO) cell lines with the highest production rate of recombinant glycoproteins remains a big challenge during early stages of cell line development. Therefore, a combinatorial titer- and TTC-based ranking is proposed, allowing for selection of distinct clones with both, high integral viable cell density (IVCD) and high specific productivity features, respectively. Using two different suspension cultivation vessels, we demonstrate that titer- or TTC-based ranking gives rise to the selection of clones with similar volumetric productivity in batch cultures. The selection of clonally-derived Chinese hamster ovary (CHO) cell lines with the highest production rate of recombinant glycoproteins remains a big challenge during early stages of cell line development.

Ivcd cell culture